Clinical study of ZEEN COLLAGEN

CSR3463 - METHODS AND RESULTS

This clinical study was double-blind, randomized, against placebo and carried on 60 healthy female subjects (mean 56 .o.). The objective was to evaluate the anti-aging potential of dailyoral intake of 5g of ZEN® fish collagen peptides on cutaneous measures and itstolerance. 5g ofZEEN®was mixed with liquid and ingested inthe morning. Thisstudy was conducted by CPCAD (independent clinical pharmacology center specialized indermatology from Nice, France) and biomechanical measurements of volunteers were controlled by certified dermatologists. Thisclinical study was performed according to French regulatory competent authority (ANSM and approved by the local Ethical Committee (CPP). Consent was obtained from each subject before entry in the study.

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Skin relief - Visia® C R

Skin relief – Visia® C R Photographies ofthe face were taken using a standardized device, the VISIA® CR. This device allows totake pictures using standardized lighting: white light, cross polarized light, parallel polarized light and UVA light. The replica was litwith incident light at 35° which produced shadowing behind each wrinkle.

Evolution of clinical score of wrinkles

Figure 1 – The wrinkles are evaluated using an 11-point clinical scale (Lemperlé: range from 0 to 10).

Elasticity - Cutometer SEM 575

Cutometer® SEM 575 by COURAGE & KHAZAKA was used to measure a deformation perpendicular to the skin during 2 seconds, followed by a relaxation period of2 seconds. This method measures the degree of deformation and the time required for the skin to return to its original state. The deformation induced on the skin is measured by an optical system. The measured parameters are: Ue, the elastic deformation; Ur, the elastic back deformation; Uf the total extensibility of the skin; Ua, the total deformation recovery; Ur/Ue the pure elasticity.

Decrease of the FOREARM skin extensibilty (Uf)as a resultof a firmer forearm skin, p<0,05

Figure 2 – This figure shows the decrease of the FOREARM skin extensibilty (Uf)with ZEEN® 1000 and ZEEN® BPMG as a result of a firmer forearm skin.

Decrease of the last minimum amplitude R4 on the BELLY skin as a higherbelly skin firmness, p< 0,05

Figure 3 – This figure shows the decrease of the last minimum amplitude R4 on the BELLY skin as a higher belly skin firmness.

Residual deformation at the end of the measuringcycle on the face skin, R9(p<0,05)

Figure 4 – The decrease of residual deformation on the FACE skin at the end of the measuring cycle on the face for ZEEN® 1000, ZEENI® HPMG.

Tolerance

It was evaluatedthrough a clinical examination and volunteer’s questionnaire. ZEEN® presents globally a good tolerance.

Conclusion

The results of oral ingestionof 5g ZEEN® on a daily basis, for 8 weeks, clinically show that regular intake of Z E N ® Fish collagen peptides should improve skin firmness and reduces wrinkles. They may also indicate apreventive effect of ZEEN® products on the skin dehydration due to cold outside conditions.